Objective:To evaluate the laboratory performance of a standardized gradient-based sperm preparation system designed to improve progressive motility recovery during density gradient centrifugation for intrauterineinsemination (IUI). Design:Retrospective, multicenter laboratory cohort study. Patients:A total of 13,416 semen samples processed for IUI. Interventions:Comparison between a conventional density gradient centrifugation method and a standardized gradient-based preparation system (RS system)...Read More

In animals, mature sperm are stored in males before ejaculation and sometimes in females before fertilization. Sperm storage provides evolutionary advantages; however, storage can also cause sperm deterioration owing to post-meiotic sperm senescence (PMSS). Yet, the extent of such deterioration, the mechanisms driving it and its fitness consequences remain poorly understood. We perform a meta-...Read More

One-third of infertile couples struggle to conceive despite normal examination and tests. For men, semen analysis is the only widely-adopted diagnostic tool to assess male infertility. However, it is unable to detect abnormal sperm function or accurately predict pregnancy except in cases of complete absence of sperm. Sperm function tests (SFT) could help by directing...Read More

The mammalian spermatozoon neck is a unique structure that functions during spermatid differentiation and spermatozoa swimming, and its contents are critical for post-fertilization embryogenesis. Mutations in proteins localizing to the neck connecting piece (the modified pericentriolar material) result in acephalic spermatozoa. In contrast, mutations in proteins localizing to the centriole often produce abnormal tail morphology....Read More

According to these extensive Danish data, if your total motile sperm count is <5 × 106/ml you can expect to live for 77.6 years, whereas if you are lucky enough to possess a count of >120 × 106/ml, your life expectancy will increase to 80.3 years. This is encouraging for those so endowed, although they will still be dying 3 years before...Read More

Are the prospective reproductive outcomes in RPL couples related to sperm DNA fragmentation index (DFI) measured by SCSA, morphology and concentration at referral? Design A prospective cohort study including 95 couples seen between 01/04/2018 and 01/12/2019 at the tertiary Copenhagen RPL Unit, Copenhagen University Hospital, Rigshospitalet and Hvidovre Hospital, Denmark. The couples had experienced three...Read More

The role of sperm DNA fragmentation index (DFI) in investigating fertility, embryonic development,and pregnancy is of academic interest. However, there is ongoing controversy regarding the impactof DFI on pregnancy outcomes and the safety of offspring in the context of Assisted ReproductiveTechnology (ART). Sperm DFI has an impact on both miscarriage rates and birth weight in...Read More

Are the prospective reproductive outcomes in RPL couples related to sperm DNA fragmentation index (DFI) measured by SCSA, morphology and concentration at referral? Design A prospective cohort study including 95 couples seen between 01/04/2018 and 01/12/2019 at the tertiary Copenhagen RPL Unit, Copenhagen University Hospital, Rigshospitalet and Hvidovre Hospital, Denmark. The couples had experienced three...Read More

The integration of artificial intelligence (AI) and deep learning algorithms into medical care has been the focus of development over the last decade, particularly in the field of assisted reproductive technologies and in vitro fertilization (IVF). With embryo morphology the cornerstone of clinical decision making for IVF, the field of IVF is highly reliant on...Read More

In a recent Fertility and Sterility review, researchers summarize the available evidence on the application of artificial intelligence (AI) and machine learning in sperm selection. The success rates of ART have remained relatively low globally due to the lack of proper sperm selection. Despite technological advancements, the final sperm selection is primarily performed manually by an embryologist following...Read More